Transgenic Plants - Methods and Protocols

di Humana Press
Stato: Nuovo
148,79 €
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Humana Press Transgenic Plants - Methods and Protocols
Humana Press - Transgenic Plants - Methods and Protocols

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Consegna: tra giovedì 2 giugno 2022 e lunedì 6 giugno 2022
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Descrizione

In 2010 the global area of transgenic crops reached 148 million hectares, an 87-fold increase since 1996, making it the most rapidly adopted technology in the history of modern agriculture. In Transgenic Plants: Methods and Protocols, Second Edition expert researchers in the field provide key techniques to investigate production and analysis of transgenic plants.  Focusing on selection and detection methods, transformation technology, gene targeting, silencing and directed mutation, metabolic engineering and pharming, the book encompasses protocols relating to major crops and model plants being used for genomic analysis. Written in the highly successful Methods in Molecular Biology™ series format, the chapters include the kind of detailed description and implementation advice that is crucial for getting optimal results in the laboratory.


 


Thorough and intuitive, Transgenic Plants: Methods and Protocols, Second Edition aids scientists in the continuous improvements being made for the production and analysis of transgenic plants.

Ulteriori informazioni

Illustrations Note:
62 SW-Abb., 30 Farbabb., 16 Tabellen
Indice:

Contents


 


Preface


 


Contributors


 


Part I. Selection AND DETECTION methods


 


1. Employment of Cytokinin Vectors for Marker-Free and Backbone-Free Transformation


 


      Craig M. Richael and Caius M. Rommens


 


 


 


2.         Organophosphorus Hydrolase; a Multifaceted Plant Genetic Marker Which is Selectable, Scorable, and Quantifiable in Whole Seed


 


T. Scott Pinkerton, James R. Wild, and John A. Howard


 


 


 


3.         Use of Northern Blotting for Specific Detection of Small RNA Molecules in Transgenic Plants


Basel Khraiwesh


 


 


 


Part II. Transformation Technology – Lower Plants


 


4.         Genetic Transformation of the Model Green Alga Chlamydomonas reinhardtii


 


Juliane Neupert, Ning Shao, Yinghong Lu, and Ralph Bock


 


 


 


Part III. Transformation Technology – Rice


 


5.     A High Efficiency Agrobacterium-mediated Transformation System of Rice (Oryza sativa L.)


 


Kenjirou Ozawa


 


 


 


6.         Selection of Transgenic Rice Plants Using a Herbicide Tolerant Form of the Acetolactate Synthase Gene


 


Masaki Endo, Tsutomu Shimizu and Seiichi Toki


 


 


 


7.         Visual Selection in Rice—a Strategy for the Efficient Identification of Transgenic Calli Accumulating Transgene Products


 


Hiroaki Saika, Haruko Onodera and Seiichi Toki


 


 


 


8.         Characterization of Rice Genes using Heterologous a Full-length cDNA Expression System


 


Mieko Higuchi, Youichi Kondou, Masaki Mori, Takanari Ichikawa, Minami Matsui


 


 


 


9. Bioactive Bead-mediated Transformation of Plants with Large DNA Fragments


 


Naoki Wada, Joyce A. Cartagena, Naruemon Khemkladngoen, and Kiichi Fukui


 


 


 


Part IV. Transformation Technology – Cereals and Other Monocots


 


 


 


10.       Agrobacterium-mediated Transformation of Sorghum bicolor Using Immature Embryos


 


Songul Gurel, Ekrem Gurel, Tamara I. Miller, and Peggy G. Lemaux


 


 


 


11.       Split-transgene Expression in Wheat


 


Mario Gils, Myroslava Rubtsova, and Katja Kempe


 


 


 


12.       Agrobacterium-mediated Transformation of Brachypodium Distachyon


 


Vera Thole and Philippe Vain


 


 


 


13.       Transformation of Barley (Hordeum vulgare L.) by Agrobacterium tumefaciens Infection of In Vitro Cultured Ovules


 


Inger Bæksted Holme, Henrik Brinch-Pedersen, Mette Lange and Preben Bach Holm


 


 


 


14.       Biolistic Mediated Production of Transgenic Oil Palm


 


Ghulam Kadir Ahmad Parveez and Bohari Bahariah


 


 


 


15.       Transformation of Oil Palm using Agrobacterium tumefaciens


 


Abang Masli Dayang Izawati, Ghulam Kadir Ahmad Parveez, and Mat Yunus Abdul Masani


 


 


 


Part V. Transformation Technology – Dicots


 


16.       Highly Efficient Transformation Protocol for Plum (Prunus domestica L.)


César Petri, Ralph Scorza, and Chinnathambi Srinivasan


 


 


 


17.       Co-transformation of Grapevine Somatic Embryos to Produce Transgenic Plants Free of Marker Genes


 


Manjul Dutt, Zhijian T. Li, Sandanand A. Dhekney and Dennis J. Gray


 


 


 


18.       Initiation and Transformation of Grapevine Embryogenic Cultures


 


Sandanand A. Dhekney, Zhijian T. Li, Manjul Dutt, and Dennis J. Gray


 


 


 


19.       Development of Highly Efficient Genetic Transformation Protocols for Table Grape Sugraone and Crimson Seedless


 


Mercedes Dabauza and Leonardo Velasco


 


 


 


20.       Cotton Pistil Drip Transformation Method


 


Tianzhen Zhang and Tianzhi Chen


 


 


 


21.       Enhanced Agrobacterium-mediated Transformation of Embryogenic Calli of Upland Cotton


 


Tian-zhen Zhang and Shen-jie Wu


 


 


 


22.       Targeted Biolistics for Improved Transformation of Impatiens balsamina


 


Andy Wetten, Jean-Luc Thomas, Alina Wagiran, Tinashe Chiurugwi


 


 


 


23.       A Protocol for Transformation of Torenia


 


Ryutaro Aida


 


 


 


24.       Efficient Modification of Floral Traits by Heavy-Ion Beam Irradiation on Transgenic Torenia


 


Norihiro Ohtsubo, Katsutomo Sasaki, Ryutaro Aida, Hiromichi Ryuto, Hiroyuki Ichida, Yoriko Hayashi, and Tomoko Abe


 


 


 


Part VI. Gene Targeting, Silencing and Directed Mutation


 


25.      


 


Isam Fattash, Basel Khraiwesh , M. Asif Arif and Wolfgang Frank


 


26.     High Frequency of Single-copy T-DNA Transformants Produced after Floral Dip in CRE-expressing Arabidopsis Plants


 


Annelies De Paepe, Sylvie De Buck, Jonah Nolf, and Ann Depicker


 


 


 


27.       A Developmentally Regulated Cre-lox system to Generate Marker-free Transgenic Brassica napus Plants


 


Lilya Kopertekh, Inge Broer, and Joachim Schiemann


 


 


 


28.       Exploiting Multisite Gateway and pENFRUIT Plasmid Collection for Fruit Genetic EngineeringLeandro H. Estornell, Antonio Granell, and Diego Orzaez


 


 


29.       A One-time Inducible Transposon to Create Knockout Mutants in Rice


 


Yuh-Chyang Charng


 


 


 


30.       Marker-free Gene Targeting by Recombinase-mediated Cassette Exchange (RMCE)


 


Hiroyasu Ebinuma, Kazuya Nanto, Saori Kasahara, and Atsushi Komamine


 


 


 


31.       Targeting DNA to a Previously Integrated Transgenic Locus Using Zinc Finger Nucleases


 


Tonya L. Strange Moynahan and Joseph F. Petolino


 


 


 


32.       Double-strand Break-induced Targeted Mutagenesis in Plants


 


L. Alexander Lyznik, Vesna Djukanovic, Meizhu Yang, and Spencer Jones


 


 


 


 


 


Part VII. Metabolic EnginEering and Pharming


 


33.       Combinatorial Genetic Transformation of Cereals and the Creation of Metabolic Libraries for the Carotenoid Pathway


 


Gemma Farre, Shaista Naqvi, Georgina Sanahuja, Chao Bai, Uxue Zorrilla-López, Sol M. Rivera, Ramon Canela, Gerhard Sandman, Richard M. Twyman, Teresa Capell, Changfu Zhu, and Paul Christou


 


 


 


34.       Production of a His-Tagged Canecystatin in Transgenic Sugarcane


 


Flavio Henrique-Silva and Andrea Soares-Costa


 


 


 


35.       Plastid Transformation as an Expression Tool for Plant-Derived Biopharmaceuticals


 


Nunzia Scotti and Teodoro Cardi


 


 


 


36.       Use of a Callus-Specific Selection System to Development Transgenic Rice Seed Accumulating a High Level of Recombinant Protein


 


Yuhya Wakasa and Fumio Takaiwa


 


 


 


Part VIII. Field TESTS


 


37.       How to Grow Transgenic Arabidopsis in the Field


 


Hanna Johansson Jänkänpää and Stefan Jansson

Editore:
Dunwell, Jim M.;Dunwell
Wetten, Andy C;Wetten
Commenti:
Provides access to the most up-to-date improvements being made for the production and analysis of transgenic plants


Provides step-by-step detail essential for reproducible results


Contains key notes and implementation advice from the experts

Tipo multimediale:
Copertina morbida
Editore:
Humana Press
Lingua:
Inglese
Edizione:
Softcover reprint of the original 2nd ed. 2012
Numero di Pagine:
497
Sommario:
The 2nd edition of this Methods in Molecular Biology™ book offers methods for investigating production and analysis of transgenic plants, focused on selection and detection methods, transformation technology, targeting, silencing and directed mutation and more.

Dati Principali

Tipologia prodotto:
Brossura
Dimensioni del collo:
0.254 x 0.178 x 0.026 m; 0.962 kg
GTIN:
09781493960781
DUIN:
H3VUFJT4PQH
148,79 €
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