Transgenic Plants - Methods and Protocols

di Humana Press
Stato: Nuovo
148,79 €
IVA inclusa - Spedizione GRATUITA
Humana Press Transgenic Plants - Methods and Protocols
Humana Press - Transgenic Plants - Methods and Protocols

Ti piace questo prodotto? Condividilo con i tuoi amici!

148,79 € IVA inclusa
Solo 1 articolo rimasto Solo 1 articoli rimasti
Consegna: tra giovedì 2 giugno 2022 e lunedì 6 giugno 2022
Vendita & spedizione: Dodax

Altre opzioni di acquisto

1 offerta a 148,79 €

Venduto da Dodax

148,79 € IVA inclusa
Stato: Nuovo
Spedizione gratuita
Consegna: tra giovedì 2 giugno 2022 e lunedì 6 giugno 2022
Visualizza altre opzioni di acquisto


In 2010 the global area of transgenic crops reached 148 million hectares, an 87-fold increase since 1996, making it the most rapidly adopted technology in the history of modern agriculture. In Transgenic Plants: Methods and Protocols, Second Edition expert researchers in the field provide key techniques to investigate production and analysis of transgenic plants.  Focusing on selection and detection methods, transformation technology, gene targeting, silencing and directed mutation, metabolic engineering and pharming, the book encompasses protocols relating to major crops and model plants being used for genomic analysis. Written in the highly successful Methods in Molecular Biology™ series format, the chapters include the kind of detailed description and implementation advice that is crucial for getting optimal results in the laboratory.


Thorough and intuitive, Transgenic Plants: Methods and Protocols, Second Edition aids scientists in the continuous improvements being made for the production and analysis of transgenic plants.

Ulteriori informazioni

Illustrations Note:
62 SW-Abb., 30 Farbabb., 16 Tabellen







Part I. Selection AND DETECTION methods


1. Employment of Cytokinin Vectors for Marker-Free and Backbone-Free Transformation


      Craig M. Richael and Caius M. Rommens




2.         Organophosphorus Hydrolase; a Multifaceted Plant Genetic Marker Which is Selectable, Scorable, and Quantifiable in Whole Seed


T. Scott Pinkerton, James R. Wild, and John A. Howard




3.         Use of Northern Blotting for Specific Detection of Small RNA Molecules in Transgenic Plants

Basel Khraiwesh




Part II. Transformation Technology – Lower Plants


4.         Genetic Transformation of the Model Green Alga Chlamydomonas reinhardtii


Juliane Neupert, Ning Shao, Yinghong Lu, and Ralph Bock




Part III. Transformation Technology – Rice


5.     A High Efficiency Agrobacterium-mediated Transformation System of Rice (Oryza sativa L.)


Kenjirou Ozawa




6.         Selection of Transgenic Rice Plants Using a Herbicide Tolerant Form of the Acetolactate Synthase Gene


Masaki Endo, Tsutomu Shimizu and Seiichi Toki




7.         Visual Selection in Rice—a Strategy for the Efficient Identification of Transgenic Calli Accumulating Transgene Products


Hiroaki Saika, Haruko Onodera and Seiichi Toki




8.         Characterization of Rice Genes using Heterologous a Full-length cDNA Expression System


Mieko Higuchi, Youichi Kondou, Masaki Mori, Takanari Ichikawa, Minami Matsui




9. Bioactive Bead-mediated Transformation of Plants with Large DNA Fragments


Naoki Wada, Joyce A. Cartagena, Naruemon Khemkladngoen, and Kiichi Fukui




Part IV. Transformation Technology – Cereals and Other Monocots




10.       Agrobacterium-mediated Transformation of Sorghum bicolor Using Immature Embryos


Songul Gurel, Ekrem Gurel, Tamara I. Miller, and Peggy G. Lemaux




11.       Split-transgene Expression in Wheat


Mario Gils, Myroslava Rubtsova, and Katja Kempe




12.       Agrobacterium-mediated Transformation of Brachypodium Distachyon


Vera Thole and Philippe Vain




13.       Transformation of Barley (Hordeum vulgare L.) by Agrobacterium tumefaciens Infection of In Vitro Cultured Ovules


Inger Bæksted Holme, Henrik Brinch-Pedersen, Mette Lange and Preben Bach Holm




14.       Biolistic Mediated Production of Transgenic Oil Palm


Ghulam Kadir Ahmad Parveez and Bohari Bahariah




15.       Transformation of Oil Palm using Agrobacterium tumefaciens


Abang Masli Dayang Izawati, Ghulam Kadir Ahmad Parveez, and Mat Yunus Abdul Masani




Part V. Transformation Technology – Dicots


16.       Highly Efficient Transformation Protocol for Plum (Prunus domestica L.)

César Petri, Ralph Scorza, and Chinnathambi Srinivasan




17.       Co-transformation of Grapevine Somatic Embryos to Produce Transgenic Plants Free of Marker Genes


Manjul Dutt, Zhijian T. Li, Sandanand A. Dhekney and Dennis J. Gray




18.       Initiation and Transformation of Grapevine Embryogenic Cultures


Sandanand A. Dhekney, Zhijian T. Li, Manjul Dutt, and Dennis J. Gray




19.       Development of Highly Efficient Genetic Transformation Protocols for Table Grape Sugraone and Crimson Seedless


Mercedes Dabauza and Leonardo Velasco




20.       Cotton Pistil Drip Transformation Method


Tianzhen Zhang and Tianzhi Chen




21.       Enhanced Agrobacterium-mediated Transformation of Embryogenic Calli of Upland Cotton


Tian-zhen Zhang and Shen-jie Wu




22.       Targeted Biolistics for Improved Transformation of Impatiens balsamina


Andy Wetten, Jean-Luc Thomas, Alina Wagiran, Tinashe Chiurugwi




23.       A Protocol for Transformation of Torenia


Ryutaro Aida




24.       Efficient Modification of Floral Traits by Heavy-Ion Beam Irradiation on Transgenic Torenia


Norihiro Ohtsubo, Katsutomo Sasaki, Ryutaro Aida, Hiromichi Ryuto, Hiroyuki Ichida, Yoriko Hayashi, and Tomoko Abe




Part VI. Gene Targeting, Silencing and Directed Mutation




Isam Fattash, Basel Khraiwesh , M. Asif Arif and Wolfgang Frank


26.     High Frequency of Single-copy T-DNA Transformants Produced after Floral Dip in CRE-expressing Arabidopsis Plants


Annelies De Paepe, Sylvie De Buck, Jonah Nolf, and Ann Depicker




27.       A Developmentally Regulated Cre-lox system to Generate Marker-free Transgenic Brassica napus Plants


Lilya Kopertekh, Inge Broer, and Joachim Schiemann




28.       Exploiting Multisite Gateway and pENFRUIT Plasmid Collection for Fruit Genetic EngineeringLeandro H. Estornell, Antonio Granell, and Diego Orzaez



29.       A One-time Inducible Transposon to Create Knockout Mutants in Rice


Yuh-Chyang Charng




30.       Marker-free Gene Targeting by Recombinase-mediated Cassette Exchange (RMCE)


Hiroyasu Ebinuma, Kazuya Nanto, Saori Kasahara, and Atsushi Komamine




31.       Targeting DNA to a Previously Integrated Transgenic Locus Using Zinc Finger Nucleases


Tonya L. Strange Moynahan and Joseph F. Petolino




32.       Double-strand Break-induced Targeted Mutagenesis in Plants


L. Alexander Lyznik, Vesna Djukanovic, Meizhu Yang, and Spencer Jones






Part VII. Metabolic EnginEering and Pharming


33.       Combinatorial Genetic Transformation of Cereals and the Creation of Metabolic Libraries for the Carotenoid Pathway


Gemma Farre, Shaista Naqvi, Georgina Sanahuja, Chao Bai, Uxue Zorrilla-López, Sol M. Rivera, Ramon Canela, Gerhard Sandman, Richard M. Twyman, Teresa Capell, Changfu Zhu, and Paul Christou




34.       Production of a His-Tagged Canecystatin in Transgenic Sugarcane


Flavio Henrique-Silva and Andrea Soares-Costa




35.       Plastid Transformation as an Expression Tool for Plant-Derived Biopharmaceuticals


Nunzia Scotti and Teodoro Cardi




36.       Use of a Callus-Specific Selection System to Development Transgenic Rice Seed Accumulating a High Level of Recombinant Protein


Yuhya Wakasa and Fumio Takaiwa




Part VIII. Field TESTS


37.       How to Grow Transgenic Arabidopsis in the Field


Hanna Johansson Jänkänpää and Stefan Jansson

Dunwell, Jim M.;Dunwell
Wetten, Andy C;Wetten
Provides access to the most up-to-date improvements being made for the production and analysis of transgenic plants

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts

Tipo multimediale:
Copertina morbida
Humana Press
Softcover reprint of the original 2nd ed. 2012
Numero di Pagine:
The 2nd edition of this Methods in Molecular Biology™ book offers methods for investigating production and analysis of transgenic plants, focused on selection and detection methods, transformation technology, targeting, silencing and directed mutation and more.

Dati Principali

Tipologia prodotto:
Dimensioni del collo:
0.254 x 0.178 x 0.026 m; 0.962 kg
148,79 €
Utilizziamo i cookie sul nostro sito Web per rendere la tua visita più efficiente e più user-friendly. Per assicurarti di poter utilizzare tutte le funzioni presenti, fai clic su "accetta cookie". Per ulteriori informazioni, consulta la nostra Informativa sulla privacy.