Immunosenescence - Methods and Protocols

di Springer US
Stato: Nuovo
130,21 €
IVA inclusa - Spedizione GRATUITA
Springer US Immunosenescence - Methods and Protocols
Springer US - Immunosenescence - Methods and Protocols

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130,21 € IVA inclusa
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Consegna: tra venerdì 8 luglio 2022 e martedì 12 luglio 2022
Vendita & spedizione: Dodax

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130,21 € IVA inclusa
Stato: Nuovo
Spedizione gratuita
Consegna: tra venerdì 8 luglio 2022 e martedì 12 luglio 2022
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Descrizione

In this volume, expert researchers detail protocols for evaluating the protean effects of immunosenescence on innate and adaptive immune function, including approaches to the analysis of antigen receptor repertoire. In addition, Immunosenecence: Methods and Protocols guides readers through cell biology and biochemical methods for analyses of telomere dysfunction, autophagy, protein oxidation, microRNAs and DNA methylation. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.


Authoritative and practical, Immunosenecence: Methods and Protocols provides a mixture of basic and advanced protocols that will be useful for immunologists in general and investigators in aging biology in particular.

Ulteriori informazioni

Illustrations Note:
XI, 270 p. 49 illus., 27 illus. in color.
Indice:

1 Isolation of Lipid Rafts from Human Neutrophils by Density Gradient Centrifugation


Carl Fortin and Tamas Fülöp


 


2 Flow Cytometry Analysis of NK Cell Phenotype and Function in Ageing


Raquel Tarazona, Carmen Campos, Alejandra Pera, Beatriz Sanchez-Correa and Rafael Solana


 


3 Flow Cytometric Identification of Fibrocytes in the Human Circulation


Xinyuan Hu, Erin M. DeBiasi, and Erica L. Herzog



 


4 Experimental Approaches to Tissue Injury and Repair in Advanced Age


Aleah L. Brubaker, Stewart R. Carter, and Elizabeth J. Kovacs


 


5 Multicolor Digital Flow Cytometry Used in Human Translational Immunology


Samit Joshi, Subhasis Mohanty, Albert C. Shaw


 


6 Flow Cytometry-Based Methods to Characterize Immune Senescence in Nonhuman Primates


Christine Meyer, Kristen Haberthur, Mark Asquith and Ilhem Messaoudi


 


7 Multiparameter Phenotyping of Human PBMCs Using Mass Cytometry


Michael D. Leipold, Evan W. Newall, and Holden T. Maecker


 


8 Imaging Immunosenescence


Feng Qian and Ruth R. Montgomery


 


9 Activation-induced Cytidine Deaminase and Switched Memory B Cells as Predictors of Effective In Vivo Responses to the Influenza Vaccine


Daniela Frasca, Alain Diaz, and Bonnie B. Blomberg


 


10 Analyzing the Effects of Aging on CD8+ T Cell Phenotype Using Flow Cytometry


Min Sun Shin and Insoo Kang


 


11 Cell-Mediated Immune Response to Influenza

Using Ex Vivo Stimulation and Assays of Cytokine and Granzyme B Responses


Janet C. McElhaney and Beth Gentleman


 


12 Assays for Monitoring Macroautophagy Activity in T Cells


Yair Botbol and Fernando Macian


 


13 Fluorescence-Based Approaches for Quantitative Assessment of Protein Carbonylation, Protein Disulfides and Protein Conformation in Biological Tissues


Ashish R. Chauduri, Rochelle Wei, Arunabh Bhattacharya, and Ryan Hamilton


 


14 Monitoring the DNA Damage Response at Dysfunctional Telomeres


Rekha Rai and Sandy Chang


 


15 Single-Cell Analysis of T Cell Receptor ab Repertoire


Pradyot Dash, George C. Wang, and Paul G. Thomas


 


16 Assessment of B Cell Repertoire in Humans


Yu-Chang Wu, David Kipling, and Deborah Dunn-Walters


 


17 Laboratory and Data Analysis Methods for Characterization of Human B Cell Repertoires by High-throughput DNA Sequencing


Chen Wang, Yi Liu, Krishna M. Roskin, Katherine J.L. Jackson, Scott C. Boyd


 


18 Discovery of Novel MicroRNAs in Aging C. Elegans


Alexandre de Lencastre and Frank Slack


 


19 Analysis of DNA Methylation by Pyrosequencing


Colin Delaney, Sanjay K. Garg, and Raymond Yung


 

Editore:
Shaw, Albert C.;Shaw
Commenti:
Includes cutting-edge methods and protocols


Provides step-by-step detail essential for reproducible results


Contains key notes and implementation advice from the experts

Tipo multimediale:
Copertina rigida
Editore:
Springer US
Lingua:
Inglese
Edizione:
1st ed. 2015
Numero di Pagine:
270

Dati Principali

Tipologia prodotto:
Libro rilegato
Dimensioni del collo:
0.264 x 0.181 x 0.022 m; 0.809 kg
GTIN:
09781493929627
DUIN:
83KH8SPFQCS
130,21 €
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