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Two Hybrid Technologies - Methods and Protocols

di Humana Press
Stato: Nuovo
191,76 €
IVA inclusa - Spedizione GRATUITA
Humana Press Two Hybrid Technologies - Methods and Protocols
Humana Press - Two Hybrid Technologies - Methods and Protocols

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191,76 € IVA inclusa
Solo 1 articolo rimasto Solo 3 articoli rimasti
Consegna: tra 2021-04-27 e 2021-04-29
Vendita & spedizione: Dodax EU

Descrizione

Protein–protein interactions (PPIs) are strongly predictive of functional relationships among proteins in virtually all processes that take place in the living cell. Therefore, the comprehensive exploration of interactome networks is one of the major goals in systems biology. The aim of Two Hybrid Technologies: Methods and Protocols is to provide a compendium of state-of-the art protocols for the investigation of binary PPIs with the classical yeast two-hybrid (Y2H) approach, Y2H variants and other in vivo methods for PPI mapping. Divided into two convenient sections, the first gives a survey of protocols that are currently employed for Y2H high-throughput screens by different expert labs in the field. Rather than detailing the principles of screening, which have been described previously, the focus is on different implementations of Y2H interactome mapping. The second section of the book considers innovative PPI detection methods that have the potential to emerge as alternative high-throughput methodologies. Written in the highly successful Methods in Molecular Biology™ series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.


 


Authoritative and accessible, Two Hybrid Technologies: Methods and Protocols  supplies researchers with a comprehensive toolbox for the identification of biologically relevant protein interactions.


Ulteriori informazioni

Illustrations Note:
35 schwarz-weiße und 27 farbige Abbildungen, 14 schwarz-weiße Tabellen
Remarks:

Includes cutting-edge methods and protocols


Provides step-by-step detail essential for reproducible results


Contains key notes and implementation advice from the experts


Tipo multimediale:
Buch
Editore:
Humana Press
Lingua:
Englisch
Numero di Pagine:
329
Sommario:
This Methods in Molecular Biology™ book offers state-of-the art protocols for the investigation of binary rotein–protein interactions (PPIs), including the classical yeast two-hybrid (Y2H) approach, Y2H variants and other in vivo methods for PPI mapping.

Dati Principali

Tipologia prodotto:
Hardback book
Dimensioni del collo:
0.262 x 0.184 x 0.024 m; 0.821 kg
GTIN:
09781617794544
DUIN:
PPVL0KT6AUR
MPN:
33786067
191,76 €
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